Abstract

UTILIZATION OF CLOVE OIL TO REDUCE SOME BIOCHEMICAL DISTURBANCES INDUCED BY HIGH FAT DIET IN RATS

Naglaa E. Mohamed* and Mervat M. Anwar

ABSTRACT

Twenty four male albino rats were divided into four groups. Group 1 (control) fed on a standard diet, group 2 was administered orally clove oil (200 mg/kg b.wt/day), group 3 fed on high fat diet (HFD) by adding saturated palm oil to the standard diet (15 g oil /100 g diet) and group 4 was administered orally clove oil + high fat diet for 60 days. Thiobarbituric acid reactive substance (TBARS), glutathione (GSH) content and catalase activities (CAT) were estimated in cardiac and hepatic tissues. Serum lipase, total lipids, phospholipids, cholesterol, triglycerides, lactate dehydrogenase (LDH), creatinine kinase (CK), Insulin, homeostasis insulin resistance and plasma glucose were determined. Also, serum free thyroxin (FT4) and free triiodothyronine (FT3) were estimated. Serum tumor necrosis factor-α (TNF-α) and 8- hydroxyl deoxyguanosine (8-OHDG) were measured, and histological examination of cardiac and aortic tissues of rats were observed. The obtained results of rats fed on HFD showed a significant increase in TBARS and significant decreases in GSH content and CAT activities in cardiac and hepatic tissues and significant decrease in serum lipase. Significant increases in serum total lipids, phospholipids, cholesterol, triglycerides, glucose, LDH, CK, insulin and homeostasis insulin resistance were recorded. Significant decreases in serum FT4 and FT3 were recorded. Significant increases in serum TNF-α and 8-OHDG were recorded. The histological examination of cardiac and aortic tissues of rats fed on HFD showed some degenerative cells in theses tissues. Conclusion: It could be concluded that the administration of clove oil to rats ameliorated the hazardous effects of high-fat diet.

Keywords: High-fat diet, Clove oil, Lipid profile, DNA oxidative stress, Tumor necrosis factor-?, Thyroid hormones, Insulin.