Abstract

VALIDATION OF A SCORE BASED ON SERUM METABOLOMES FOR EARLY BREAST CANCER DIAGNOSIS

Mohamed Omran, Ramzy Rashed*, Faten Zahran, Hossam Darwish and Arafa A. Belal

ABSTRACT

Back ground: Early detection of breast cancer is helpful for diagnosis, treatment, enhancing patient survival and reducing death rates. Because of the low diagnostic sensitivity and specificity of widely used breast cancer biomarkers, this study aimed to suggest useful biomarkers for early detection of breast cancer based on metabolomes profile. Material & Methods: Serum samples were obtained from 120 females served as a malignant group, 47 females served as a benign group and 55 females served as a healthy control group. Serum selected metabolomes (8-hydroxy-2’-deoxyguanosine, 1-methylguanosine, and 1-methyl adenosine) were detected and quantified by gas chromatography-mass spectrometry. Cutoff values of individual and combined metabolomes with CA15-3 were analyzed using the receiver operating characteristics to test the efficiency of candidate metabolisms and identify patients with breast cancer. Results: Cutoff values of CA15-3, 8-hydroxy-2’-deoxyguanosine, 1-methylguanosine and 1-methyl adenosine were 30.5 U/l, 15.0 μg/l, 18.5 μg/l and 22.0 μg/l respectively. Diagnostic performance analyses of individual and combined metabolomes with CA15-3 gives significant values of the area under the curve ranged from 0.74 to 0.94 with good efficiency 88.8%. There were significant correlations between the candidate metabolomes and clinicopathological throughout the different patients with breast malignant diseases. Conclusion: candidate metabolomes are suggested to be useful biomarkers for the early detection of breast cancer.

Keywords: Metabolomics; Mass Spectrometry; Metabolomes; Breast cancer; CA15-3.