OVERVIEW ON DOWNSTREAM PROCEDURES FOR ENZYME PRODUCTION
Michele Vitolo*
ABSTRACT
Enzymes can be purified from microorganisms and animal and plant tissues by downstream unit operations such as filtration, centrifugation, sedimentation, flocculation, coagulation, cell disruption, extraction, precipitation, chromatography, crystallization, and drying. Depending on the desired enzyme purity, different combinations of unit operations can be used. The final enzyme product must be formulated adequately to meet the requirements of industry and clinical analysis laboratories, which may demand for a powdered or liquid enzyme preparation.
Keywords: Enzymes, purification, production, downstream.
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