Abstract

IN-VITRO AND IN-VIVO EVALUATION OF ANTI-SNAKE VENOM ACTIVITY OF LEAVES OF AMPELOCISSUS INDICA LINN. PLANCH

S. Venkatesh*, Shinju V. and Sreelakshmi S.S.

ABSTRACT

The objective for the current study was to investigate the in-vitro and in-vivo evaluation of Anti-snake venom activity of leaves of Ampelocissus indica Linn. Planch. The air dried powdered leaves of Ampelocissus indica Linn. Planch. was extracted with ethanol by using the Soxhlet extractor. The prepared extract was subjected to acute toxicity study, in-vitro and in-vivo evaluation of Anti-venom activity and effect of bleeding time in mice. Acute toxicity study was carried out according to the OECD guidelines 425. The in-vivo neutralization Anti-venom activity of extract was performed on Swiss Albino mice. Preliminary phytochemical screening showed the presence of Alkaloids, Flavonoids, Tannins, Proteins and Amino acids, Sterols and Triterpenoids. The results of acute toxicity study revealed that the Ampelocissus indica Linn. Planch. Leaves extract was safe till 2000mg/kg body weight. The 200, 400 and 600 mg/kg of ethanolic extract of leaves of Ampelocissus indica Linn. Planch. significantly neutralizes the lethality (up to 3 folds) induced by the Naja naja venom in both in-vitro and in-vivo Anti-venom activity. The study indicates that the plant Ampelocissus indica Linn. Planch. leaves possess promising Anti-snake venom activity.

Keywords: Ampelocissus indica Linn. Planch; Leaves; Ethanolic extract; Acute oral toxicity; Anti-venom activity.