Abstract

ANTIDIABETIC, ANTIHYPERLIPIDEMIC, ANTIOXIDATIVE EFFECTS OF RIDA HERBAL BITTERS IN STREPTOZOTOCIN-INDUCED DIABETIC MALE WISTAR RATS

Ajao Folasade Omobolanle*, Oyadeyi Ayodele Stephen and Abiola Samad

ABSTRACT

This study aimed at investigating antidiabetic, antihyperlipidaemic and antioxidant profile of RIDA herbal bitters in diabetic male Wistar rats. Thirty-two (32) male Wistar rats were randomly divided into control and experimental groups of n = 8. The experimental rats were administered intraperitoneal single dose of freshly prepared streptozotocin (50 mg/kg b.w) dissolved in 0.1M citrate buffer (pH 4.5) after 12 h fasting period. On the establishment of diabetes, food consumption was monitored daily. Fasting blood glucose level and body growth rate were determined on 1st, 3rd and 7th day. Biomarkers of oxidative stress, lipid peroxidation markers kidney and liver function tests were determined post treatment. Pancreas were excisedfor histological evaluation. The administration of RIDA bitters significantly reduced (p<0.05) TC, LDL, TG, and significantly increase (p<0.05) HDL-C in the RIDA and GMP treated groups compared to STZ group. There were significant increases (p<0.01) in enzyme activity of SOD, GPx, GSH, CAT and significant reduction (p<0.01) of MDA concentration in RIDA treated group compared to STZ group comparable to GMP. Urea, uric acid, and creatinine concentrations were significantly reduced (p<0.01) after RIDA treatment compared to STZ group. In addition, AST, ALT, ALP were significantly (p<0.01) reduced in the RIDA group compared to STZ which were comparable to the GMP. Conclusion: This result indicates that RIDA bitters has antidiabetic, antihyperlipidemic and antioxidative properties that could be useful in the management of diabetes mellitus.

Keywords: Diabetes mellitus; RIDA bitters; Hyperglycaemia; Oxidative stress; Lipid profile.