Abstract

EFFECT OF ROSEMARY ON GLUCOCORTICOID INDUCED OSTEOPOROSIS IN RATS

*Nawal Al-Bader, Soheir Al-Masri and Raghad Alfayz

ABSTRACT

Osteoporosis is common bone disease leads to increase risk of bone fracture. The purpose of this study was to evaluate the effect of rosemary in different forms (leave powder, leave extract and oil) on glucocorticoid (dexamethasone) induced osteoporosis in rats. Fifty female white albino rats were classified into five groups. First group kept as negative control while others injected with glucocorticoid (dexamethasone) at dose of 7mg/kg of b wt intramuscularly once a week all over period of the experiment (60 days) to induce osteoporosis in rats. These rats divided into positive group and 3 treated groups with rosemary powder (fed on 10% of rosemary powder in standard diet, rosemary extract (2.5g/Kg/day by stomach tube) and rosemary oil (5 mg/kg by stomach tube). The results showed that the osteoporotic rat groups treated with rosemary powder, extract andoil showed significant higher of the nutritional values (body weight gain &FER) however increased the lowering values of bone mineral density and concentration and serum Ca and P along with reduced the elevation of osteocalcin and could normalize femur ash weight, calcium and phosphorus. Consumption of rosemary oil could elevate ALP and normalized ALT & AST enzymes activity Moreover, treated rat with rosemary powder and extract could lower the elevation of renal function parameters compared to control positive group. In conclusion, powder,extract and oil of rosemary have anti osteoporotic activity in glucocorticoid induced osteoporosis in rats. The study recommended that intake of rosemary in any form is useful for patient administer glucocorticoid and suffer from osteoporosis.

Keywords: Osteoporosis, Glucocorticoid, Rosemary leaves- Blood and Bone analyses- Rats.