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World Journal of Pharmacy and
Pharmaceutical Sciences

( An ISO 9001:2015 Certified International Journal )

An International Peer Reviewed Journal for Pharmaceutical and Medical Research and Technology

Impact Factor : 8.025

ICV : 84.65

WJPPS Citation

	All	Since 2019
Citation	5450	3969
h-index	23	20
i10-index	134	84

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WJPPS: NOVEMBER ISSUE PUBLISHED

NOVEMBER 2024 Issue has been successfully launched on 1 NOVEMBER 2024.

Abstract

DEVELOPMENT AND VALIDATION OF DIFFERENCE SPECTROPHOTOMETRIC METHOD FOR THE DETERMINATION OF SECNIDAZOLE IN BULK AND TABLET DOSAGE FORMS

Roa’a M. Osman, Sitalnafer A. Dafaalla, Shahd S. Siddig, Wafa N. Ahmed and Dr. Mohammed E. Adam*

ABSTRACT

A simple, accurate, and sensitive spectrophotometric method has been developed and validated for determination of Secnidazole in bulk and tablet dosage forms. The developed method was based on the measurement of difference in absorbance of the drug in acidic (0.1M HCl) and basic (0.1M KOH) medium at 278nm and 313nm, respectively. Constructed calibration curve showed linear relationship (R2=0.999) in the range of 5-30μg/mL. The limits of detection and quantification were 1.11μg/ml and 3.36μg/ml, respectively. Precision of the method was satisfactory reflected by the low RSD% values (˂ 2%). The percentage recoveries at three different levels were found 101.2, 99.6 and101.3%; n=3.

Keywords: Secnidazole; Difference Spectrophotometry; Acidic; Basic; Validation.

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