Abstract

ACUTE AND 28 DAYS REPEATED DOSE ORAL TOXICITY STUDIES OF CLASSICAL SIDDHA POLYHERBAL FORMULATION ASHUWATHI CHOORANAM IN WISTAR RATS AS PER OECD GUIDELINES

Dr. Devaki R.*, Kalpana R., Santhosh Kumar R., Rajamma Devi, Velpandian V.

ABSTRACT

The science of the ancient Siddhars are far advanced and beyond the scope of modern material scientists. The aim of the present study is to evaluate the acute and 28 days repeated oral toxicity studies of Siddha polyherbal formulation Ashuwathi Chooranam from the classical Siddha literature, in wistar albino rats was conducted as per OECD guidelines. The experimental protocol was authorized by the institutional ethical committee (IAEC) under CPCSEA. The studies were carried out in healthy wistar albino female rats weighing 200–220 gms. A limit test at one dose level of 2000mg/kg body weight will be carried out with 6 animals (3 animals per step) and it does not produce any mortality in animals. The dose level of 5, 50, 250 and 500mg/kg body weight was administered stepwise. Observations were made and recorded as per the guideline after substance administration and found to be no major changes in skin and fur, and mucous membranes, and also circulatory, autonomic and central nervous systems, and somatomotor activity and behavioural patterns. The body weight the animals from the treated group showed a gain in body weight. The 28 days repeated dose oral toxicity study in haematological and biochemical parameters revealed that there are no remarkable changes with respect to the control group except the lymphocytes, platelets and the PCV and except the HDL that slightly increased with dosage of 500mg/kg and no notable changes in Urea, Creatinine, uric acid and SGPT, SGOT, ALP, T-protien and bilirubin levels.

Keywords: Acute toxicity, sub-acute toxicity, Ashuwathi Chooranam, Siddha, Wistar albino rats, OECD guidelines.