Abstract

ANTI-HYPERGLYCEMIC AND ANTI-OXIDANT EFFECT IN STREPTOZOTOCIN-INDUCED DIABETIC RATS BY BAUHINIA VARIEGATA FLOWER EXTRACT

Prateek Pandey*, Shradha Pandey and Alok Mukerjee

ABSTRACT

To evaluate the anti-hyperglycemic activity of ethanolic extract of Bauhinia variegata flower (BVE) using streptozotocin (STZ) model and anti-oxidant activity of BVE evaluated by in-vitro methods. Ethanolic extract of Bauhinia variegata flower (BVE) 200 and 400 mg/kg body weight was administered orally according to different groups of animals once a daily for 21 days. Blood glucose levels were estimated at 0, 7th, 14th and 21st day by glucometer (one-touch) and on 21st day biochemical parameters (lipid profile) and histopathological examination of isolated organ (pancreas) was also estimated. The anti-oxidant activity of BVE was performed by in-vitro methods by help of DPPH scavenging activity and hydrogen peroxide scavenging (H2O2) assay. A significant reduction in blood glucose was observed in diabetic animals treated with BVE at different doses when compared with diabetic rats. Levels of triglycerides, total cholesterol, low density lipoprotein (LDL), very low density lipoprotein (VLDL) were decreased while administrating BVE at different doses, compared with their control values in diabetic animals. Also a significant anti-oxidant activity was observed in selected methods for reducing power and scavenging DPPH and H2O2 radicals. The results show that BVE possesses significant anti-hyperglycemic and anti-oxidant activity. The anti-hyperglycemic activity might be attributed to stimulating effect on glucose utilization and anti-oxidant property.

Keywords: Bauhinia variegata; DPPH scavenging activity; hydrogen peroxide scavenging (H2O2) assay; anti-hyperglycemic; streptozotocin.