Abstract

EVALUATION OF NEPHROPROTECTIVE ACTIVITY OF ANDROGRAPHOLIDE IN EXPERIMENTAL ANIMAL (GENTAMICIN INDUCED NEPHROTOXICITY).

Amol Vilas Supekar* and Prof. H. J. Pagar

ABSTRACT

Andrographolide is a labdane diterpenoid produced by Andrographispaniculata, (AP) a predominant constituent of at least 26 Ayurvedic formulations used to treat liver ailments, nephrotoxicity. The plant produces a range of bioactive components like andrographolides and polyphenols. Among these, andrographolide are pharmacologically the most active compounds; however, andrographolide content in conventionally propagated or wild plants is evaluated for Nephroprotective activity. From the toxicity studies 100 mg/kg, 200mg/kg and 400 mg/kg dose was selected for the Nephroprotective activity. The Gentamicin induced method is employed for the assessment of the nephroprotective activity &to check the nephrotoxicity In the present study, Cystone (500mg/kg) served as positive control given by Oral route and the Andrographolide were given by oral route. Na+, K+ and cl- ion concentration in urine were determined by using autuanalyser. The treatment of Gentamicin and Andrographolide at 400 mg/kg has increased the urine output significantly, urinary concentration of Na+, K+ and Cl- ions were the parameters of the study. The Gentamicin and Andrographolide at 400mg/kg b.w. has shown good nephroprotective activity, whereas100mg/kg, 200 mg/kg dose has shown moderate nephroprotective activity. From the present study it may be concluded that the Andrographolide has nephroprotective activity.

Keywords: Andrographolide (andrographis panniculata), Cystone, Na+/K+ ratio, Nephrotoxicity index.