METHOD DEVELOPMENT AND VALIDATION FOR TAZOBACTAM AND PIPERACILLIN IN COMBINE PHARMACEUTICAL DOSAGE FORMS BY RP-HPLC
R. Sai Vinay* and N. Anjaneyulu
ABSTRACT
A new method was established for simultaneous estimation of a Tazobactamand Piperacillin by RP-HPLC method. The chromatographic conditions were successfully developed for the separation of Tazobactamand Piperacillin by using Inertsil -ODS C18 (250 x 4.6 mm, 5μ), flow is 1.0 ml/min, mobile phase ratio was Methanol: Buffer (80:20), detection wave length was 225 nm.
Keywords: Tazobactam and Piperacillin, RP-HPLC, Acetonitrile.
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