Abstract

PREPARATION AND EVALUATION OF NANOEMULSION CONTAINING ANTIBACTERIAL DRUG

Km. Seema*, Abhinav Agarwal, Munendra Mohan Varshney, Pankaj Kumar Sharma

ABSTRACT

The topical treatment of skin infection has proven to be very beneficial as the systemic load of active pharmaceutical ingredient and their related side effect are reduced when compared to other routes of administration. The application of drug to skin surface avoids the major fluctuation of plasma level typical for repeated administration of rapidly eliminated drug. This route of administration circumvents the first pass metabolism of drug and decrease gastrointestinal side effect of drug administered through oral route. Nanoemulsion system has been developed not only to enhance percutaneous absorption but to allow the drug targeting on the skin or even to its substructure. They have been reported to enhance the topical permeation of drugs significantly compared to conventional formulations such as solution, gels and cream. Mupirocin is a topically used antibiotic from a strain of Pseudomonas fluorescens. It has shown excellent activity against gram-positive staphylococci and streptococci. Method: High Speed Homogenizer used for the decrease the particle size of nanoemulsion optimized formulation. Result: The drug absorption of mupirocin drug in Phosphate Buffer 221nm and Methanol 219 nm. The optimized formulation was droplet size of nanoemulsion 100-500 nm, and performed Polydispersity index detection-0.659 and droplet size 64.1, Zeta potential- 34.93 mV, drug content, pH, TEM, Viscosity, Phase separation. In-vitro study drug release shows 1.54 folds bioavailability enhance of nanoemulsion of optimized batch in comparison with the marketed formulation of T-Bact cream (GSK) which shows increases the bioavailability for topically used.

Keywords: Nanoemulsion, Surfactant, Co-Surfactant, Bioavailability, Mupirocin.