Abstract

SCREENING OF IN VITRO ANTIMICROBIAL ACTIVITY OF BRYOPHYLLUM PINNATUM, VICIA FABA, PHASEOLUS VULGARIS, CYAMOPSIS TETRAGONOLOBA AND IT’S SYNERGISTIC ACTIVITY WITH ANTIBIOTIC AND HONEY

Pooja Kapadne* and Kishori S. Tarfe

ABSTRACT

Nature has been source of medicinal agents since ancient time. The importance of herbs in the management of human ailments have an great importance. Plant kingdom has an unlimited storage of invaluable active ingredient for a number of diseases. Traditional healing systems around the world that utilize antimicrobial properties of herbs are an important source of discovery of new antimicroboial agents for treatment of drug resistant strains. In this study the antimicrobial activity of few plant extracts such as Bryophyllum pinnatum, Vicia faba (Broad beans), Cyamopsis tetragonoloba (cluster beans), Phaseolus vulgaris (common bean or French beans) was evaluated with selected microorganisms such as Escherichia coli ,Staphylococcus aureus, Salmonella typhi by agar cup method. In addition, the possible synergistic effects of these plant extracts with antibiotics and honey was studied. There was remarkable inhibition of the bacterial growth by the these plant extracts. The antimicrobial activity of the Bryophyllum pinnatum, Vicia faba (Broad beans), Phaseolus vulgaris(common bean or French beans) is due to presence of various secondary metabolite no synergistic activity of combination was observed. Hence these plants can be used to discover bioactive natural products that may serve as leads in the development of new pharmaceutical activities. As antimicrobial activity of extract has shown the promising effect so the MIC and MLC should be determined so that effective concentrations for intake or local application can be determined. We should try different combinations of extract with other antibiotics and honey or we should use different concentration of extract to obtain a synergistic activity.

Keywords: Medicinal agents, antimicrobial activity, plant extacts, agar cup method, synergistic activity.