Abstract

PHARMACOGNOSTICAL AND EXPERIMENTAL EVALUATION OF MALLIKA (JASMINUM SAMBAC (LINN.) AIT.) WITH SPECIAL EMPHASIS ON BREAST CANCER CELL LINE

*Dr. Priya Pramod Wadate

ABSTRACT

In the plant kingdom, there are number of plants that yield medicines useful to mankind. Jasminum sambac (Linn.) Ait. Family – Oleaceae, called Mallika in Sanskrit is well known glabrous twining shrub widely grown in gardens throughout the India. Breast cancer is a type of cancer originating from breast tissue, most commonly from the inner lining of milk ducts or the lobules that supply the ducts with milk.According to V.G.Desai and Kirtikar & Basu Mallika acts on the female reproductive organs (uterus and breast). The flowers of Mallika act as „Lactifuge‟, at the puerperal state, in the case of breast abscess. J. sambac flowers and leaves of Mallika are largely used in folk medicine to prevent and treat breast cancer. The current in vitro anticancer study was conducted with an aim to check its anticancer effect on MCF-7, MDA-MB-231 breast cancer cell lines, at concentrations 10-640μg/ml. The MTT assay protocol was followed to observe the activity of the study drug. The study drug was tested in 96 well plates and methanolic and aqueous extract of leaf and flower of mallika were used in different dose levels. As per MTT assay protocol, methanolic extract had shown significant activity on MCF-7 breast cancer cell line and had shown negligible activity on MDA-MB231.

Keywords: Jasminum sambac linn. Ait, breast cancer, MCF-7, MDA-MB-231 and MTT assay, in vitro study.