Abstract

MECHANISM BASED PROTECTIVE EFFECT OF DELPHINIUM DENUDATUM ON CISPLATIN INDUCED NEPHROTOXICITY

Siddique NA, Ahmad S, Mujeeb M*

ABSTRACT

Introduction and Aim: In present investigation in-vitro and In-vivo antioxidant and nephroprotective activities of aqueous root extract of Delphenium denudatum were evaluated. Materials and Methods: Free radical-scavenging activity of aqueous roots extract was carried out by DPPH HPLC method. The in-vitro nephroprotective study was investigated by GGT assay (γ-glutamyl transpeptidase) method. In-vivo antioxidant and nephroprotective potential of aqueous root extract of Delphenium denudatum (300,600 and 800 mg kg-1) were screened in animal rats model. Oxidative stress and nephrotoxicity was induced by cisplatin (5mg kg-1 bw). Results: Aqueous root extract of Delphenium denudatum showed 83.38% inhibition where as 92.67% inhibition was produced by standard reference (ascorbic acid). The IC50 of root extract was found to be 189.3 where as IC50 61.29 was produced by standard drug mesna (Sodium 2-mercaptoethanesulfonate) in GGT assay. In this study the aqueous root extract of Delphenium denudatum at a dose of (300,600 and 800 mg kg-1) showed significant dose-dependent reduction in the elevated blood urea, uric acid, serum creatinine and normalized the histopathological changes. However, oxidative stress was noticed in renal tissue as evidenced by a significant decrease in glutathione level, superoxide dismutase and catalase activities, also a significant increase in malondialdehyde levels when compared to control group. The animal in the curative regimen showed good response to the aqueous extract at 800 mg kg-1 bw. Conclusion: It is concluded that the aqueous root extract of Delphenium denudatum possesses nephroprotective and antioxidant activity and could offer a promising role in the treatment of renal injury caused by nephrotoxins like cisplatin.

Keywords: Delphenium denudatum, Nephroprotective activity, GGT, Cisplatin, Phenolics compounds, Flavonoid compounds