![]() |
|||||||||||||
|
All | Since 2019 | |
Citation | 5450 | 3969 |
h-index | 23 | 20 |
i10-index | 134 | 84 |
DEVELOPMENT AND VALIDATION OF RP-HPLC AND UV-SPECTROSCOPIC METHOD OF ECONAZOLE IN BULK AND PHARMACEUTICAL DOSAGE FORM
Neha R. Patil*, Sunil P. Pawar and Vipul H. Jain
ABSTRACT An Analytical method development and its validation is an important aspect in drug discovery process. a new, simple and rapid reversed-phase high-performance liquid chromatography (HPLC) method and UV-Spectroscopic method were developed and validated for the determination of Econazole in bulk and pharmaceutical dosage form. The UV- spectroscopic determination was done at an absorption maximum of 260 nm. A linear response was observed in the range of 2-10μg/ml with regression coefficient of 0.997. The method was then validated for different parameters as per the ICH guidelines. RP-HPLC method was performed by using a mobile phase consisting mixture of acetonitrile : water 0.05%PH 3 Adjusted with OPA in the proportion 70:30v/v. (Cosmosil) C18 column (4.6mm x 250mm,5μm) used as a stationary phase. HPLC analysis of Econazole was carried out at a wavelength of 260 nm with a flow rate of 0.7ml/min. The linear regression analysis data for the calibration curve showed good relationship with coefficient 0.999. The method was validated for different parameters as per the guidelines. Keywords: Econazole, method development, RP-HPLC, UV-Spectroscopic, validation. [Download Article] [Download Certifiate] |