Abstract

GAS CHROMATOGRAPHY FOR ESTIMATION OF MENTHOL, THYMOL, CAMPHOR, TURPENTINE OIL, NIAOULI OIL AND EUCALYPTUS OIL IN VAPORIZING OINTMENT

Nief Rahman Ahmad*, Fawaz K. Ibrahim and Mohammad J. Essa

ABSTRACT

development and validation of a novel, simple, fast gas chromatography–flame ionization detection (GC–FID) method for simultaneous estimation of turpentine oil, niaoli oil, eucalyptus oil, camphor, menthol and thymol in vaporizing ointment(Smicks Ointment). Good separation of turpentine oil (Rt=3.651), niaoli oil (Rt= 4.350), eucalyptus oil (Rt=5.451), camphor(Rt= 8.238), menthol (Rt= 9.433) and thymol (Rt= 14.511). There is no method available for simultaneous estimation of turpentine oil, niaoli oil, eucalyptus oil, camphor, menthol and thymol, there is no limitation of sample composition. There is no difference in a results obtained from analysis of commercial Smicks Ointment samples when compared with those obtained with standard reference materials for simultaneous estimation. Good separation of six materials which separated at about 3.651, 4.350, 5.451, 8.238, 9.433and 14.511 min respectively on a capillary column with nitrogen(3.3 mL/min) as carrier gas. Noninterference of any peak with the peaks of interest which confirms the selectivity of this method. The relative standard deviation of the method was less than 1.9% and accuracy (average recovery) was 100±1.6%. The method was successfully applied to analysis of vaporizing ointment.

Keywords: GC, Turpentine, Niaoli, Eucalyptus oil, Camphor, Menthol, Thymol.