Abstract

AMELORATIVE EFFECT OF CURCUMIN AGAINST AMIKACIN TOXICITY IN ALBINO RATS

*Ashraf A. A. Elkomy, Faten I. Elsayed, Mohammed E. Abd El-Gayed and Doaa EL Sayed Gazal

ABSTRACT

Due to great hazard effects of antibiotic the following study aimed to investigate the adverse effect of amikacin in biochemical. oxidative status beside histological and immunohisto chemical examination of Liver and kidney tissue as well as the protective effect of curcumin. Tweenty four male Wister albino rats were randomly divided into main four groups including:- G (1): Served as control group and it includes six rats, they were adminstered 0.5ml of saline orally for 21 consecutive days. G (2): it includes six rats, they were administered 200mg/kg curcumin orally for 21 consecutive days. G (3): it includes six rats, they were adminstered 7.14 mg/kg body weight/day of amikacin intraperitonially for 21 consecutive days. G (4): it includes six rats, they were administered 200mg/kg curcumin orally concurrently with 7.14 mg/kg body weight/day of amikacin. Results revealed that amikacin induced significant increases in liver and kidney function parameters including AST, ALT, ALP. creatinin, and urea as well as decrease in total potein level. Moreover, marked an increase in malondialdehyde (MDA) and decreases in glutathione (GSH) and catalase (CAT), Superoxide dismutase (SOD) levels that indicate oxidative stress levels along with enhanced activated caspase-3 expression in the hepatic, and renal tissues following amikacin adminsteration. On the benifacial side oral adminsteration of of curcumin at the dose 200 mg/kg for 21 days significantly mitigate theses toxic effects. So it is concluded that curcumin has great hepatorenal, antioxidant, antiopoptotic effect.

Keywords: Amikacin, Curcumin, Liver, Kidney.