Abstract

IN-VITRO COMPERATIVE STUDY OF THREE ORNAMENTAL PLANTS LEAVES EXTRACTS FOR IMMUNOMODULATORY ACTIVITY

Kore H. Rudramuni* and Manish S. Kondawar

ABSTRACT

In this Present research work the effects of Ethanolic extracts of leaves of Adina cordifolia (Roxb). Hook, Nyctanthes arbor-tristis Linn and Thevetia peruviana (Pers.) Roxb were investigated on the functions of peritoneal macrophages In-vitro studies. Macrophage functions were determined through Nitric oxide (NO) estimation and phagocytic activities NBT reduction test. The results indicated that ethanolic extracts of leaves of Adina cordifolia (Roxb). Hook, Nyctanthes arbor-tristis Linn and Thevetia peruviana (Pers.) Roxb were enhanced nitric oxide (NO) production at 50 μg/ml whereas it phagocytic activity of the macrophage was enhanced by the ethanolic extracts at a dose of 100 μg/ml. The proliferation was significantly increased by ethanolic extracts in the EC50 value of 32.27 μg/ml and 49.06 μg/ml after 48 hours incubation. Collectively the results demonstrated that ethanolic extracts of all three plants enhanced innate as well as adaptive immune response and proved the immunostimulating potential of the plants.

Keywords: Adina cordifolia, Nyctanthes arbor-tristi, Thevetia peruviana, Immunomodulation, Macrophage, Lymphocyte proliferation.