Abstract

ANALYTICAL METHOD DEVELOPMENT & VALIDATION OF A METHOD FOR THE QUANTITATION STUDIES OF PRAZIQUANTEL AND PYRANTELPAMOATE IN BULK AND PHARMACEUTICAL DOSAGE FORM BY USING RP-HPLC

Rajendra G. Shete, Dr. N. S. Dighe* and K.V. Dhamak

ABSTRACT

The Present manuscript describes a new simple, specific, precise and accurate reverse phase high performance liquid chromatography method for the RP-HPLC for quantitation studies for praziquantel and pyrantel pamoate in bulk dosage form. The quantitation studies were carried out by using C18 (250 x4.6 mm) colum with mobile phase contaning methanol: water in thre ratio of80:20 buffer PH was maintained at 4.5 adjusted with 0.01m orthophosphoric acid, which is pumped at a flow rate of 0.8ml/min. For UV detection was monitored at 217nm. The peaks obtained were sharp with retention time of praziquantel and pyrantel pamoate. 5.0 min and 6.59 min respectively. The Calibration Curves were linear (R2=0.999) over the concentration from 5-10ml for praziquantel and pyrantel pamoate. The percentage recoveries of praziquantel and pyrantel pamoate were found to be in the range of 98.06 and 99.98 percent respectively. The proposed method was validated and successfully applied to the quantitation studies of praziquantel and pyrantel pamoate in combined bulk dosage forms. The Calibration Curves were linear (R2=0.999) over the concentration from 5-10 ugml for praziquantel and pyrantel pamoate respectovely. The percentage recoveries of praziquantel and pyrantel pamoate were found to be in the range of 98.06 and 99.98 percent respectively. The proposed method was validated and successfully applied to the quantitation studies of Praziquantel and Pyrantelpamoate in combined bulk dosage form respectively.

Keywords: Praziquantel, Pyrantel pamoate, Quantitation Studies, RP-HPLC, UV Spectroscopy.