Abstract

DEVELOPMENT AND VALIDATION OF U.V-SPECTROPHOTOMETRIC AND HPLC METHOD FOR HYDROCHLOROTHIAZIDE IN BULK AND TABLET DOSAGE FORM

Rajesh D. Ahire*, Vipul H. Jain, Sunil P. Pawar, Azam Z. Shaikh, Amit R. Dhankani

ABSTRACT

The aim of this research work was to develop simple, precise and rapid UV-Spectrophotometric and HPLC methods for analysis of Hydrochlorothiazide in Bulk and Tablet dosage form. UV-Spectrophotometric method was developed for the estimation of Hydrochlorothiazide in individual tablet dosage form. The proposed methods were applied for the determination of drugs in bulk and tablet dosage form. Determination of Hydrochlorothiazide was done by simple method. In this method, concentration was obtained by using the absorptivity values calculated for the drugs at wavelengths, 260.0 nm. A rapid and reliable HPLC method was developed and validated for estimation of Hydrochlorothiazide in bulk and tablet dosage form. Chromatographic separation was performed on Agilent C 18 (4.6 mm  250 mm i.d.), 5µm particle size in isocratic mode, using a mobile phase Methanol: Water (30:70 v/v), pH 7. The retention time for Hydrochlorothiazide was found to be 2.59 min. The method was applied to marketed tablet formulations. The UV-Spectrophotometric method was found to be simple, economical and rapid as compared to HPLC. But, HPLC method was found to be more accurate, precise, robust and rugged. Both these methods can be used for routine analysis of Hydrochlorothiazide in Bulk and Tablet dosage form.

Keywords: Hydrochlorothiazide; UV-Spectrophotometric; HPLC; Tablet.