Abstract

DETAIL PHYTOCHEMICAL STUDY OF APHANAMIXIS POLYSTACHYA (WALL) PARKER

Shweta S. Saboo, Rani W. Chavan*, G.G.Tapadiya, S.S.Khadabadi

ABSTRACT

the dried stem bark of aphanamixis polystachya (wall)parker was successive extracted with petroleum ether, chloroform, methanol solvents for 48 hours in soxhlet apparatus and solvents from extracts was evaporated under vacuum. all three extracts were subjected to phytochemical investigation and HPLC analysis. these herbal plants have the phyto constituents such as phenyl compounds, coumarins, essential oils, terpenoids, monoterpenoids, diterpenoids, triterpenoids, steroids, alkaloids and other nitrogenous compounds. the plant taken for this study is aphanamixis polystachya (wall) parker. it is called rohituka in sanskrit, malampuluvan in tamil. this plant belongs to meliaceae family. this plant is used for liver disorders, rheumatoid arthritis, leucorrhoea and anthelmintic ulcers,anticancer.this paper shows that high performance liquid chromatography (hplc) is a suitable analytical method for determining beta sitosterol and stigmasterol in nhexane fraction of aphanamixis polystachya(wall) parker stem bark methanolic extract.std beta sitosterol and stigmasterol showed retension time 6.12 and 11.05 min repectively,90% acetonitrile and 10% water the retension time of n-hexane fraction of methanolic extract of plant 6.16 , and 11.03 min repectively.

Keywords: beta sitosterol , stigma sterol, HPLC.