Abstract

QUANTITATIVE DETERMINATION OF NIACIN IN AQUEOUS EXTRACT OF OCIMUM SANCTUM BY HIGH PERFORMANCE LIQUID CHROMATOGRAPHY

C. T. Sadashiva*, H. M. Firoz Hussain, S. Nanjundaiah, R. Patil, R. Ashok, M. Sudheer, Y.D.Pavithra and P. Arunagiri

ABSTRACT

Niacin (C6H5NO2), also known as nicotinic acid is one of the precious human nutrient and a form of B3 vitamin group available in herbs. The quantitative estimation of these organic acids in the solvent extract is very crucial and the methodology of the identification and quantification were distinctly different due to its various chemical properties. The present study was designed to evaluate the presence of niacin in the aqueous extract of Ocimum sanctum. L by reverse phase high performance liquid chromatography (RP-HPLC). RP-HPLC, a simple, sensitive and precise methodology has been developed, validated and used for quantitative determination of niacin from the aqueous extract of O. sanctum. L. The RP-HPLC was carried out followed by the aqueous extraction of O. sanctum. The C18 (250mm x 4.6mm, 5 μm) column and a mobile phase comprising of 0.05M Potassium Dihydrogen phosphate acetonitrile (65:35) which was obtained by trial and error method at a flow rate of 0.7 ml /min in an isocratic system. The detection of niacin was carried out at 240 nm. The results were concluded as the higher amount of niacin was present in the aqueous extract of O. sanctum. Further studies are warranted to exhibit the various bio-protective effects of O. sanctum

Keywords: Ocimum sanctum, Niacin, chromatography, RP-HPLC, C18 column.