Abstract

BIO-MIMETIC VESICLES ENCAPSULATING IRINOTECAN HYDROCHLORIDE ALONG WITH HERBAL BIO POTENTIATORS FOR BIOAVAILABILITY ENHANCEMENT: PREPARATION, CHARACTERISATION AND EVALUATION

Gandhi Kinjal B.* and Dr. P Ajithkumar

ABSTRACT

The present study was aimed to prepare sustained release Biomimetic vesicles of irinotecan Hydrochloride alone (R1 to R8) and along with bioenhancers (BP1 to BP7) by thin film hydration technique using span 40 as surfactant, cholesterol as membrane stabilizing agent, Genistein and Quercetin as Drug bioavailability enhancers and dicetyl phosphate (DCP) as charge inducing agent. All the formulations of Vesicles were characterized on the basis of physical appearance and entrapment efficiency. The in-vitro release studies of optimized formulation of Irinotecan Hydrochloride alone and along with efficiency improvers were performed and compared with pure drug released. The entrapment efficiency of irinotecan hydrochloride in optimized formulation of Biomimetic Vesicles containing Irinotecan along with Efficiency improvers was found to 59.9 % and entrapment efficiency of Genistein and Quercetin was found to be 30.30% and 60.1%respectively. In vitro drug release of optimized formulations of Irinotecan Hydrochloride without and with bio potentiators was found to be 99.77% and 60.11% at the end of 12 h respectively. Results concluded that Vesicles of Irinotecan containing bioenhancers followed sustain release pattern.

Keywords: Irinotecan Hydrochloride, Thin Film Hydration Technique, Entrapment efficiency, Invitro study, Genistein, Quercetin.