Abstract

PHYTOCHEMICAL ANALYSIS AND ANTIBACTERIAL ACTIVITY OF CISSAMPELOS PAREIRA LIN. RHIZOME EXTRACT AGAINST SOME BACTERIAL STRAIN

Laxmi Shrestha* and Surendra Prasad Gupta

ABSTRACT

The plant Cissampelos pareira belonging to Menispermaceae family contains various phytoconstituents with specific value in ethnomedicinal practices which is significant in the field of health care system. The study had been envisioned to evaluate phytochemical and antimicrobial activity of plant’s rhizomes with aim to analyze the potentiality of this plant as new potent antibacterial agents. For phytochemical screening; Mayer’s reagent test, Baljet reagent test, Gelatin test, Spot test, etc were used and analyzed. In our research, the methanolic and ethyl acetate extract was prepared using the plant’s rhizomes by double maceration extraction method and their antimicrobial activity was assessed against Staphylococcus aureus & Escherichia coli organisms using the standard antimicrobial agents such as Ciprofloxacin & Ampicillin by disc diffusion method. Based on phytochemical screening, the plant extracts contain alkaloids, glycoside, fixed oil, carbohydrate, etc. The methanolic extract showed the highest yield value (8.5552%) in comparison to ethyl acetate extract (3.2872%). The methanolic extracts showed more positive tests for presence of phytoconstituents. Similarly, after analyzing the Zone of inhibition, it was found that Ethyl Acetate extract was more sensitive against S. aureus in comparison to methanolic extract whereas the methanolic extract in turn was more sensitive against E. coli than Ethyl acetate extract. Following the broth dilution method, the minimum inhibitory concentration of methanolic extracts against S. aureus & E. coli was 1.25 mg/ml & 0.56 mg/ml and of ethyl acetate extracts were 0.62 mg/ml & 1.25 mg/ml. The study of various bioactive substances from our research work recommends its potential use in formulation of antimicrobial agents for the treatment of many ailments.

Keywords: Cissampelos pareira, Phytochemical screening, Antimicrobial activity, Disc diffusion method, Zone of inhibition.