Abstract

ANTIDIABETIC POTENTIAL OF ETHYL ACETATE EXTRACT OF ADHATODA VASICA NEES ON HIGH FAT DIET AND STREPTOZOTOCIN INDUCED DIABETIC RATS

Vadivelan R.*, Gopala Krishnan R. and Arun T.

ABSTRACT

The present study is to investigate the antidiabetic effect of the ethyl acetate extract of Adhatoda vasica Nees on type 2 diabetes induced by high –fat diet and streptozotocin in diabetic male Wistar albino rats. Diabetes is a costly disease with high financial burden. This study is to treat the diabetes using natural resources. The hypoglycemic effect of ethyl acetate extract of Adhatoda vasica Nees was assessed by -using high fat diet (HFD)/ streptozotocin(STZ). The diabetic rats were randomly divided into four groups and treated orally by gavage with vehicle, ethyl acetate extract (200 mg/kg body weight and 400 mg/kg body weight), glibenclamide (10 mg/kg body weight) respectively, T2DM Induction through 3weeks of high fat diet (HFD) intervention was followed by single low dosage of STZ (30mg/kg dissolved in 0.1 mol/L citrate buffer at pH 4.5,i.p). Diabetic rat models showed a significant increase in blood glucose level as compared to the treated animal which was determined by OGTT.T2DM rats have decreased levels of liver glucose 6 phosphate dehydrogenase (G6PD), glutathione reduced (GSH), nitric oxide (No),and antioxidant enzymes. Furthermore, the present study has shown the hypoglycemic, hypolipidemic, and antioxidant the ethyl acetate extract of Adhatoda vasica Nees as confirmed by its ability for ameliorating most of the alteration caused in the studies parameters of diabetic rats.The ethyl acetate extract of Adhatoda vasica Nees may be useful as therapy against oxidative stress and liver damage in type2 diabetes mellitus and is therefore recommended for further studies.

Keywords: T2DM, Ethyl acetate extract of Adhatoda vasica Nees, hyperglycemia, Lipid profile, Liver toxicity, Oxidative stress.