Abstract

IDENTIFICATION OF FATTY ACIDS AND FATTY ACID ESTERS FROM ETHYL ACETATE BARK EXTRACT OF HOLOPTELEA INTEGRIFOLIA (ROXB.) PLANCH BY GC-MS

Amir Hassan*, Munawwer Rasheed, Mohsin Ali, Sohail Hassan, Urooj Nazim, Ghazala Ishrat, Kanwal Hussain and Mansoor Ahmed

ABSTRACT

It is an ornamental plant with certain medicinal characteristics due to many valuable and active Phytoconstituents in various parts of the plant. Ethyl acetate extract of the bark was triturated with warm n-hexane and warm n-hexane fractions were pooled in order to get Holoptelea integrifolia Bark-Ehyl Acetate-Hexane Soluble (HIB-EA-HS) which was analyzed on GC-MS. The identified fatty acids and fatty acid esters included: octadecanoicacid, methyl ester (1), hexadecanedioic acid,dimethyl ester (2), eicosanoic acid, methylester (3), octadecanedioic acid, dimethyl ester (4), Cis-13-eicosenoic acid (5), docosanoicacid, methyl ester (6), 1,2-benzenedicarboxylicacid, diisooctyl ester (7), methyl- 21-methyldocosanoate (8), and tetracosanoicacid, methyl ester (9). Not any saturated fatty acid was found in the present study. A number of fatty acids and fatty acid esters have been identified from the stem extract of H. integrifolia but these fatty acids and fatty acid esters were identified the first time from the bark of the plant.

Keywords: Fatty acids, Fatty acid esters, bark, Holoptelea integrifolia, GC-MS.