Abstract

EFFECTS OF EGYPTIAN ANNONA SQUAMOSA LEAVES EXTRACTS AGAINST ALLOXAN INDUCED HYPERGLYCEMIA IN RATS

Dina M. El-Baz*, Z. El-Khayat and Ahmad K. Hssan

ABSTRACT

The objective of the present study was to investigate the hypoglycemic effect of ethanolic and aqueous extracts of Egyptian Annona squamosa leaves and mix of them against alloxan induced hyperglycemia in rats. The study was performed on 60 male albino rats divided into 6 groups. Healthy rats served as control group, diabetic group, Vildagliptin drug treated diabetic group, aqueous extracts of Annona squamosa leaves treated diabetic group, ethanolic extracts of annona squamosa leaves treated diabetic group and mix extracts of Annona squamosa leaves treated diabetic group. Experimental diabetes was induced by single intraperitoneal injection of 150 mg/kg body weight alloxan. After one month blood samples were collected for assessment of blood glucose, insulin, lipid profile (triglycerides, total cholesterol, high density lipoprotein, and low density lipoprotein, albumin, total protein, urea, creatinine and the activities of diagnostic marker liver enzymes aspartate aminotransferase, alanine aminotransferase, the antioxidant properties of the extract were determined by tissue nitric oxide, reduced glutathione and malondialdehyde. Sections of liver and pancreas organs were qualitatively examinated under light microscope. The result showed that mean concentrations of FBS, serum cholesterol, triglycerides, low density lipoprotein, liver enzymes, serum urea and creatinine were significantly decreased and serum insulin, high density lipoprotein, albumin, total protein were significantly increased in all treated diabetic groups compared with the diabetic group. The results showed that the mix of ethanol and aqueous extracts has higher significant effect in the most biochemical markers than the individual effect of aqueous and ethanolic extracts respectively.

Keywords: Annona squamosa leaves extracts, blood glucose, diabetes mellitus, insulin, oxidative stress.