Abstract

USE OF TLC, UV, NMR, IR AND LC-MS/MS FOR PURIFICATION AND IDENTIFICATION OF ANTIBACTERIAL COMPOUNDS

Maheep Kumar*, Alok Kumar Ravi , Ravi Kumar Asthana

ABSTRACT

Cyanobacteria are known to produce array of compounds. In an earlier report, we reported antibacterial activity in methanolic crude extracts of laboratory grown Lyngbya aestuarii and Aphanothece bullosa isolated from Chilka Lake and local paddy field respectively. In this report the same methanolic crude extracts were subjected to TLC purification twice by altering the solvents and UV-illuminated bands bioassayed. Such UV illuminated potent bands obtained after 2nd TLC was subjected to spectroscopic analysis (UV, IR, 1H NMR and LCMS/MS). We have screened malyngolide from L. aestuarii however, a diterpenoid from A. bullosa.

Keywords: Lyngbya aestuarii? Aphanothece bullosa ?TLC purification spectroscopic analysis? antimicrobial.