Abstract

DEVELOPMENT AND VALIDATION OF UV-SPECTROSCOPIC METHOD FOR SIMULTANEOUS ESTIMATION OF L-GLUTATHIONE REDUCED AND ?- LIPOIC ACID IN TABLET DOSAGE FORM USING DICHLONE AS A DERIVATIZING REAGENT

P. Dhruv* and P. Patel

ABSTRACT

The present work aims at development of a novel method for the simultaneous estimation of L-Glutathione reduced and α- Lipoic acid using dichlone as a derivatizing reagent. As L-Glutathione reduced has various pharmacological uses like its activity against liver disorders, cancer and as an anti-aging agent. Similiarly, α-lipoic acid is of a major use in pain of liver disorders and diabetic neuropathy and it also increases the production of glutathione in the body, hence a proper analytical method for the fixed dose and its validation method is necessary for combined formulation. Dichlone forms a complex with both the drugs and its estimation is done in the visible region (400-800 nm) using Absorbance correction method.

Keywords: L-Glutathione reduced, ?- Lipoic acid, dichlone as a derivatizing reagent, Absorbance correction method.