Abstract

MOLECULAR AND IN SILICO STUDIES ON DHFR OF UROPATHOGENIC KLEBSIELLA PNEUMONIAE

Archana Moon*, Deeba Khan, Pranjali Gajbhiye, Monali Jariya and Amit aksande

ABSTRACT

The most common uropathogenic Gram negative bacteria are Escherichia coli and Klebsiella pneumoniae. MDR Klebsiella infection poses a significant challenge for treatment. K. pneumoniae strains have become increasingly resistant to antibiotics, rendering infection by these strains very challenging to treat. Dihydrofolate reductase (DHFR) has been a target for treatment against a variety of pathogenic microorganisms, since the folate-dependent enzymes are essential for DNA synthesis and methylation. This study was designed to study promising antibacterial molecules viz; Chlorogenic acid, Hippuric acid, Ellagic acid, Gallic acid and Quercetin as alternatives to antibiotics. Antibiotic and molecular studies on Klebsiella pneumoniae were performed. Further, molecular docking using AutoDock, an automated suite of proteinligand docking tools was used to identify potent antibacterial compounds to treat Klebsiella pneumonia mediated UTI.

Keywords: Antibacterial activity, Chlorogenic acid, Hippuric acid, Ellagic acid, Gallic acid, Quercetin, DHFR, Molecular Docking, MDR, UTI.