Abstract

ENZYMATIC ACTIVITY INDUCED BY GLYPHOSATE HERBICIDE ON THE FRESHWATER FISH CHANNA PUNCTATUS (BLOCH)

Anamika Singh* and Ajay Singh

ABSTRACT

Fishes are very sensitive to aquatic pollution as they exposed directly to toxicants discharged from different sources to water bodies. They are good bio-indicator for assessing the effect of toxicant present in water bodies. Unfortunately, the use & abuse of herbicide has surfaced that it not only lethal to target but also sub lethal to non-target aquatic organism. Accumulation of toxic substances in body of aquatic organism leads to physiological and metabolic disorder. Glyphosate at low concentration damage liver, muscle, kidney and skin cells. There was a significant (P<0.05) negative correlation between LC value of glyphosate and exposure period. In vivo exposure of fish Channa punctatus with sub lethal doses (40% and 80% of LC50 of 24h) of glyphosate for 24h or 96h, significantly alter the enzyme activity like AchE, acid and alkaline phosphstses, Lactic dehydrogenase, Transaminases (GOT & GPT) was also time and dose dependent manner.

Keywords: Glyphosate, Channa punctatus, Herbicide, Lactic dehydrogenase, Transaminases, Phosphatases, Acetylcholinesterase.