Abstract

BIOCHEMICAL AND MOLECULAR STUDY ON THE EFFECT OF MURRAYA KOENIGII (CURRY) AND MORINGA OLEIFERA ON CARDIAC MITOCHONDRIAL DYSFUNCTION IN DIABETIC RATS

Yakout A. El Senosi, Omayma A. R. Abou Zaid, Afaf Desouky A.elmaged, EL Saeed. E. Ahmed and Heba S. EL. Din Ebrahim*

ABSTRACT

In the present study, the biochemical and molecular study effect of Murraya Koenigii (curry) and Moringa oleifera aquous extract of leaves on cardiac mitochondrial dysfunction in experimentally induced diabetes mellitus in rats by Streptozotocin (STZ) was evaluated. sixty male albino rats weighting 150-200 g and divided into four equal groups of 15 rats each. Group 1:(Control group): received normal diet only. Group 2:(Diabetic rats group): rats received a single dose of Streptozotocin (STZ) (50- mg/kg-b.wt i.p). Group 3: (Diabetic rats + Murraya Koenigii (curry) aquous extract of leaves at dose of 200mg/kg/ twice daily, orally for four weeks after diabetes induction. Group 4:(Diabetic rats+ Moringa oleifera aquous extract of leaves at dose of 200mg/kg/ twice daily orally for four weeks after diabetes induction. Heart tissue was collected at the end of experiment. The obtained results revealed that, a significant increase in MDA, NO, and cytochrome C gene with marked decrease in CAT, GSH Px, SOD1 gene, PGC 1α gene and ATp1A1 gene in diabetic control rats. Treatment with Murraya Koenigii (curry) and Moringa oleifera were able to mitigate diabetic abnormalities through significant decreasing MDA, NO and cytochrome C gene but higher than normal control and significant increasing CAT, GSH Px, SOD1 gene, PGC 1α gene and ATpase but less than normal control.. These results suggest that Murraya Koenigii (curry) and Moringa oleifera act as antioxidative, anti apoptic and improve mitochondrial dysfunction so provide agood protection to the heart.

Keywords: Murraya Koenigii (curry), Moringa oleifera, STZ, Diabetes, mitochondria, antioxidant enzymes, SOD1 gene, PGC 1? gene, ATpase gene and cytochrome c gene.