Abstract

STUDIES ON AVIAN COLIBACILLOSIS IN BAHRI LOCALITY OF KHATROUM STATE, SUDAN

Abdulahi S. Rooble, Abubaker A. El Ayis*, Fadlalla E., Salih I. A. Salih, Eassa A. Eassa, Abdel-Rahman E. Elbasheir and Abdelrahim A. O. Abdalla

ABSTRACT

Forty five poultry farms located in Bahri locality of Khartoum were investigated for the presence of avian colibacillosis and the study was extended for 6 months of the years 2016 and 2017. The prevalence rate of colibacillosis in Bahry locality poultry farms was 11.1%, the prevalence rate increased with broilers poultry farms 3 (6.67%) than layers farms 2 (4.45%) and it was inversely proportionate to the age of birds. The prevalence of avian colibacillosis was higher (8.9%) in closed system farms than open system (3.2%). This is the first report of incidence rate of avian colibacillosisin Bahri locality. In this investigation a total of 92 bacterial isolates were obtained from 81 diarrhoeic samples, according to the cultural characteristics, bacterial morphology and biochemical reactions results, the identified bacteria were: Escherichia coli 59.8%, Proteus mirabilis 19.6%, Enterococcus faecalis 6.5%, Klebsiella pneumonae sub spp. ozaenae 5.4%, Salmonella spp. 3.3%, and Staphylococcus aureus 5.4%. Gram negative bacteria represented the predominant isolated bacteria (94.6%), compared to gram positive bacteria (5.4%). A total of 55 bacterial isolates were obtained from 45broilers diarrhoeic samples, According to the cultural characteristics, bacterial morphology and biochemical reactions results, the identified bacteria were: Escherichia coli 65.5%, Proteus mirabilis 16.3%, Staphylococcus aureus 9.1%, Salmonella spp. 5.5% and Klebsiella pneumonae sub spp. Ozaenae 3.6%. Gram negative bacteria represented the predominant isolated bacteria (90.9%), compared to gram positive bacteria (9.1%). A total of 37 bacterial isolates were obtained from 36layersdiarrhoeic samples, According to the cultural characteristics, bacterial morphology and biochemical reactions results, the identified bacteria were: Escherichia coli 51.4%, Proteus mirabilis 24.3%, Enterococcus faecali16.2% and Klebsiella pneumonae sub spp. Ozaenae 8.1%. Ten E. coli isolates were selected randomly and examined for their production of heat-stable enterotoxin using Suckling mouse test (SMT). Seven isolates (70%) out of 10 gave positive results with SMT test. This is the first report in Sudan using suckling mouse test (SMT) for detection of heat-stable enterotoxin (STa) in E.coli isolated from cases of avian colibacillosis.

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