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  • WJPPS NOVEMBER ISSUE PUBLISHED
  • NOVEMBER 2018 Issue has been successfully launched on 1 November 2018

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  • Its our Pleasure to Inform you that WJPPS Impact Factor has been increased from 6.647 to 7.421 due to high quality Publication at International Level

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Abstract

IN VITRO MULTIPLICATION OF HELICTERES ISORA THROUGH NODAL STEM SEGMENT EXPLANT FROM MATURE PLANT

Mahesh Chand Meena* and Ramraj Meena

ABSTRACT

Helicteres isora is an important medicinal shrub belonging to family Sterculiceae. It is commonly known as „Marorphali‟. All parts of the plant are extensively used in indigenous system of medicine. It has cytotoxic, antinociceptive, antifungal, antioxidant, antispasmodic and antibacterial activity and is, used to treat diseases like dyspepsia, colic, scabies, gastropathy, diabetes, diarrhoea and dysentery. It contains high amount of medicinally important secondary metabolites like β-sitosterol, betulinic acid, β-tocopherol, coumarins, ellagic acid etc. In Helicteres isora apospory, seed dormancy and low viability of seeds were observed. Therefore, the present work on Helicteres isora was undertaken to develop a highly efficient, reliable and reproducible protocol for their micropropagation. Effect of various basal media viz. MS-medium, B5-medium and MSB5-medium on multiple shoot proliferation were studied and it was concluded that MS-medium was best for maximum shoot proliferation. Maximum shoot bud proliferation (14.2±0.545) was observed on MS-medium fortified with BAP (1.5 mg/l) and Kn (1.5 mg/l). Therefore this medium was designated as “shoot bud induction, proliferation and elongation medium”.

Keywords: Helicteres isora, Medicinal shrub, BAP, Kn, Multiplication.


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