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  • WJPPS AUGUST ISSUE PUBLISHED
  • AUGUST 2018 Issue has been successfully launched on 1 August 2018

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Abstract

SIMULTANEOUS ESTIMATION OF MELATONIN AND HESPERIDIN BY UV SPECTROPHOTOMETRIC METHOD

K. B. Chougule*, M. B. Chougule, S. R. Devkar and P. M. Sangave

ABSTRACT

The present work aimed to develop and validate spectrophotometric methods for simultaneous estimation of melatonin and hesperidin, in combined dosage form. Method is based on solving a simultaneous equation. Absorbance of melatonin and hesperidin were measured at the respective absorbance maximum (λ max) at 274 and 298 nm. Methods are validated according to ICH guidelines. Linearity range for melatonin and hesperidin is 2-10 μg/ml and 2-10 μg/ml at respective selected wavelengths. The coefficient of correlation for melatonin at 274 nm and hesperidin at 298 nm is 0.997 and 0.993, respectively. A percentage estimation of melatonin and hesperidin from the liposome is 98.88% and 99.89% respectively, with standard deviation less than 2. The proposed method was simple, rapid, and validated and can be used successfully for the routine simultaneous estimation of melatonin and Hesperidin combined liposome formulation.

Keywords: Melatonin, hesperidin, UV spectroscopic method, Simultaneous equation method, Method Validation, ICH guidelines.


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