Abstract

PHYTOCHEMICAL SCREENING AND ANTI MICROBIAL ACTIVITY OF n-HEXANE, ETHYL ACETATE, AND METHANOL EXTRACT OF ALOE VERA L.

Shirly Kumala* and Muhammad Andi Mahdiansyah

ABSTRACT

Utilisation of plant as traditional medicine has been well documented and has become a traditional method of medicinal therapy by the Indonesian community for many decades. One of those plants was“lidah buaya”, which also known as Aloe vera (Aloe vera L.). Traditionally, Aloe vera leaves were applied in hair sculp to increase the hair growth and treatment for burns, sore throat, and diabetes. In this study, extraction of Aloe vera leaves were conducted by maceration. Three solvents, (n-hexane, ethyl acetate and methanol) were used in the extraction. The Extract was then used in antibacterial activity test with Agar diffusion methods using paper disk. Phytochemical screening by Farnsworth methods. The Phytochemical screening showed the presence of flavonoid, saponin, tannin,quinone, steroid, dan triterpenoid. The activity of antibacterial of ethyl acetate extract against Salmonella typhi (22.33±0.577mm), Staphylococcus epidermidis (19.67±0.577mm), Proteus vulgaris (23.67±0.577 mm). Antibacterial activity of the methanol extracts against (Salmonella typhi), Staphylococcus epidermidis and Proteus vulgaris 17±0, 19±0 and 18.33±.0.577 mm, respectively. Aloe vera L has broad spectrum antibacterial activity.

Keywords: Antibacterial activity, Phytochemical screening, Aloe vera L.