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Ibrahim Afsal V. T. *, Girendra Gautam, Rajasekaran S., Nishad K. M.


The present research work of Herbal Formulation With Ajwa Seed (Phoenix Dactylifera L.) Extract And Zam-Zam Water For Anticancer activity is an effective formulation from the pioneer research and discussion. As well as literature survey, the possibility of the study is fine. The parameters and methodology for the research work is made initially by plant authentifications. Phoenix Dactylifera was authentified by respective authority. The seeds of riped fruit was crushed, powdered and examined for number of evaluation studies, which includes physical characteristic studies like smell, taste, colour, solubility studies and loss on drying. The dried seeds of Phoenix Dactylifera ethanolic extract was prepared by soxhlet apparatus by using ethanol (95%v/v)as a solvent. Phyto chemical screening also performed to determine presence of active constituents. The advanced studies also performed with the help of HPTLC finger printing method for qualitative evaluation of seeds compared sample with standard, and result proved that active constituent present in the sample Phoenix Dactylifera is similar as standard. and the acute toxicity study was performed by OECD Guidelines 423 and therapeutic index was determined, which were correlated standard parameters. The cell line studies was performed by selected cell Seven cell lines HT-29 (Human colon cancer cell lines), A 431(Human skin cancer cell lines), HL-60 (Human leukemia cell lines), MCF-7 (Human breast cancer cell lines), and Human Epidermoid Larynx Carcinoma cell line (Hep 2). A 549 (Human lung cancer cell lines), HeLa (human cervical cancer) Dalton’s lymphoma cells (mouse ascites tumor) which includes MTT assay, SRB assay, LDH assay etc. The in vivo cancer studies performed in rats. the result given by all evaluation parameters are satisfactory by achieving all the experimental criteria. The optimized seed powder formulated pharmaceutically for formulations were confirmed by six trials. The invtro studies were performed with the help of analytical studies. The stability studies also performed for six month. For the formulation. zam zam water was selected as vehicle because of abundant source from natural and certain anti oxidant effect. In the light of the above points and considering the importance of natural drugs, the present work on the investigation of the plant namely Phoenix dactylifera L.Family Arecaceae reveals The generation of new therapeutic agents for the Anti-Cancer.

Keywords: Phoenix dactylifera L, HPTLC finger printing method, Zam-Zam Water, MTT assay.

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