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R. K. Sujatha and *S. Rajan


Pathogenic microorganisms are effectively controlled by making use of modern combined antibiotics. Improper and overuse of antibiotics cause various side effects and creates resistance among bacterial pathogens. To overcome these problems peoples turned towards the use of medicinal plants as therapeutic agents. Aegle marmelos fruit pulp is an efficient traditional phytomedicine used for the treatment of various infections. Antibacterial activity of aqueous and ethanolic extract of Aegle marmelos fruit pulp s were determined using disc diffusion, modified agar dilution methods. MIC, MBC, Percentage of inhibition and IC50were calculated using modified drug dilution method. The results showed that the fruit pulp extracts of A. marmelos have antimicrobial activity against E. coli, Salmonella, Shigella, Klebsiella, Pseudomonas, Staphylococcus aureus, Streptococcus pyogenes. Ethanolic extracts showed the highest inhibition zone of 16.0±1.0mm diameter against Pseudomonas aeruginosa. The plant extracts were shown to have a best MIC at 150±050μg/ml for ethanolic extract and 216.6±028.8 μg/ml for aqueous extract. Percentage of inhibition created by the extracts against the pathogens varied from 47.6% to 92. 6% 2ith best activity against Escherichia coli by both the extracts. Effective IC50 exhibited by ethanolic extract 112.9 μg/ml concentrations against E. coli.

Keywords: Bacterial pathogens, MIC, MBC, Antibacterial activity, IC50, Percentage inhibition, Aegle marmelos, Fruit pulp.

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