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Abstract

ISOLATION AND CHARACTERIZATION OF POTENT OIL DEGRADING MICROBES FROM OIL CONTAMINATED SOURCES

*Amit Pandey, Devendra Pratap Singh

R&D Division, MRD LifeSciences, Lucknow-226010, India.

ABSTRACT

The study was designed to evaluate the capability of bacterial strains to degrade oil under in vitro conditions. Two mechanic workshops within Lucknow were selected for collecting two different samples from each site. One isolate showed complete oil degradation capability within seven days incubation period. The results tend to perform certain characterization tests. In order to check expression of oil degrading gene, plasmid was isolated and transformed to E. coli cells.

Keywords: E. coli, Degradation, Crude oil.


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