Abstract

INSILICO PEPTIDE MODELING STUIDIES AND STRUCTURAL ANALYSIS ON RIBULOSE -1, 5 BISPHOSPHATE CARBOXYLASE IN GRACILARIA EDULIS

*Shoba K., Hebsibah Elsie B. and Bavyasri S.

ABSTRACT

Seaweed or algae, belongs to a group of plant-like organisms that grow in the sea. Some algae are one-celled organisms such as microalgae, They generate energy through photosynthesis. The groups (or phyta) of seaweed that are commonly consumed include: Green algae, Brown algae, Red algae and Blue green algae. The response of the marine macroalgae Gracilaria edulis (Gmelin) Silva to nutrient pulses of varying magnitude was investigated to test its applicability as a marine bioindicator at two oligotrophic locations. The protein ribulose 1,5 bisphosphate present in this algae. The enzyme is commonly referred to as rubisco. The limiting reaction within the Calvin Cycle is carried out by the enzyme Rubisco. The enzyme is located in the chloroplasts' stroma. The sequences of Rubisco retrieved from National Centre for Biotechnology Information in fasta format. The biophysical characterization and motif analysis were carried out using bioinformatics tools like protparam, scan prosite and glob plot. We used advanced peptide modeling server for methodology studies. The final results of our project clearly explain that the identified motif –peptide sequence and the 3D peptide structure are potential candidates for drug docking studies and also act as novel molecular markers useful for pharmacoinformitics and clinical endocrinology studies.

Keywords: Seaweed, Bioindicator, Rubisco, Calvin cycle, NCBI, Bioinformatics, Peptide Modeling.