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Abirami K., Monika N. and Sakthi Abirami M.*


Tuberculosis is an infectious disease, caused by the bacterium called Mycobacterium tuberculosis which is a slow growing bacteria. It is an airborne communicable disease caused by transmission of aerosolized droplets of Mycobacterium tuberculosis, affecting all the organs in the body that are rich in blood and oxygen, the lungs being most commonly affected. The multidrug resistant of bacterial and fungal strains of clinically important pathogens fetches the interest of scientist to develop newer broad spectrum antimicrobial agents. The objective of the study is to evaluate anti-tubercular and antimicrobial activity of semisynthetic compound 5- Nitro 2- thiophene carboxaldehyde. The
compound is analysed for antimicrobial activity using agar dilution and agar diffusion method & anti-tubercular activity using Microplate Alamar Blue Assay. The results shows that the 5-Nitro 2- thiophene carboxaldehyde has increased bactericidal activity against gram positive bacteria when compared to gram negative bacteria. 5-Nitro 2- thiophene carboxaldehyde at the concentration 7μg/ml seems to be effective against Staphylococcus aureus, Enterococcus, E.coli and Salmonella typhi but not against Pseudomonas aeruginosa. This compound shows minimum inhibitory concentration at 50μg/ml by preventing the colour change from blue to Pink. Change in colour from blue to pink was scored as growth. So, this compound shows the anti-tubercular activity. It concludes that 5-Nitro 2- thiophene carboxaldehyde shows antibacterial, antifungal and anti-tubercular activity.

Keywords: 5-Nitro 2- thiophene carboxaldehyde, antibacterial, antifungal, anti-tubercular, Microplate Alamar Blue Assay (MABA), agar dilution and agar diffusion

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