Abstract

PROTECTIVE EFFECT OF Mussaenda frondosa Linn EXTRACTS ON ISONIAZID INDUCED HEPATOTOXICITY IN WISTAR RATS

Dr. Sambrekar Sudhir N.* and Dr. Patil Suhas A

ABSTRACT

The present study was undertaken to investigate the protective effect and possible mechanism of alcoholic (AlE) and aqueous extract (AqE) from Mussaenda frondosa Linn leaf (MF) on Isoniazid (INH)-induced hepatic injury in Wistar rat. Hepatotoxic parameters studied in vivo include serum transaminases (AST, and ALT, ALP), bilirubin, protein, lipid profile (Cholesterol, triglyceride, VLDL and HDL) and level of antioxidants together with histopathological examination. Liv 52® was used as a reference hepatoprotective agent (5ml/kg-1b.w.). AlE and AqE (200 mg/kg-1b.w.) on oral administration decreased the level of AST, ALP, ALT, bilirubin, cholesterol, triglyceride, VLDL, MDA and increased the level of protein, HDL and antioxidants (SOD, GSH and CAT) in rats being treated with isoniazid (INH). Pentobarbitone -induced sleeping time study was carried out to verify the effect on microsomal enzymes. Histopathological observations confirmed the beneficial roles of MF against INH-induced liver injury in rats. Possible mechanism may involve their antioxidant activity.

Keywords: Mussaenda frondosa Linn, hepatoprotective, Isoniazid, Liv 52®, sleeping time.