IN VITRO REGULATION OF ADIPOGENIC DIFFERENTIATION OF ADIPOSE-DERIVED MESENCHYMAL STEM CELLS
Shaymaa H. El-Arabi, Mohamed A. Sobh, Ahmed M. El-Waseef and Amr Negm*
ABSTRACT
Human adipose tissue acts as a practical supply of mesenchymal stem
cells (hAMSCs) compared to other donor sites. So our attention was
directed to study the adipogenic differentiation capacity of
mesenchymal stem cells derived from human adipose tissue.
Extraction of MSCs from human adipose tissue were done. After that
we were studied the in vitro characterization of MSCs clustar
differentiation(CD) surface marker. Moreover, we tested the ability of
it to form fibroblast-like colony and differentiated into adipogenic
tissues by special identification markers. Some identification tests have
been done such as: viability test, Fibroblast-Like Colony- Forming
Unit Assay and CD surface marker to assure that we have correctly
obtained mesenchymal stem cells. These hAMSCs expressed CD29, CD 90, CD 105 and
CD13 but not CD14 and CD34. RT-PCR of adipogenic differentiated hAMSCs expressed
Lipoprotein Lipase, Leptin , Adiponectin, peroxisome proliferator-activated receptor-γ and a
transcription factor known to be involved in control of adipocytic differentiation at different
time intervals after 4, 7, 14 and 21 days. HAMSCs have the ability to proliferate into
monolayer culture and multilineage adipogenic differentiation as a result of treating with
inductive conditions and thus have potential clinical applications in regenerative medicine.
Keywords: Human Adipose Tissue, Mesenchymal Stem Cells, Surface Marker, Differentiation, Adipogenesis and Gene Expression.
[Download Article]
[Download Certifiate]