DEVELOPMENT AND VALIDATION OF REVERSE PHASE HPLC METHOD FOR SIMULTANEOUS ESTIMATION OF DICYCLOMINE HYDROCHLORIDE, MEFENAMIC ACID AND PARACETAMOL IN TABLET DOSAGE FORM
S.R. Lokhande, S.M. Mhetre, S.S. Pekamwar*, T.M. Kalyankar
School of Pharmacy, Swami Ramanand Teerth Marathwada University, Nanded-431606. India.
ABSTRACT
A simple, rapid and reliable RP-HPLC method has been developed for
qualitative and quantitative estimation of Dicyclomine Hydrochloride
(DCL), Mefenamic Acid (MEFE) and Paracetamol (PARA) in bulk
drug and pharmaceutical formulations using a mobile phase consisting
mixture of methanol and 1% acetic acid (80:20 v/v) at pH 4.3 and at
flow rate of 1.2 ml/min at 400 C temperature. An ODS C-18 (Hypersil
5μ, 250mm x 4.6 mm, i.d.) column was used as stationary phase. The
retention time of Dicyclomine Hydrochloride, Mefenamic Acid and
Paracetamol were 7.1 min., 4.4 min and 2.1 min. respectively.
Linearity was observed at concentration range of 2000-4500 μg/ml for
Dicyclomine Hydrochloride, 25-150 μg/ml for Mefenamic Acid and
50-300 μg/ml for paracetamol. Percent recoveries obtained for
Dicyclomine Hydrochloride, Mefenamic Acid and Paracetamol were
99.63, 99.71and 99.78 respectively. The proposed method is precise,
accurate, selective and rapid for the simultaneous determination of
Dicyclomine Hydrochloride, Mefenamic Acid and Paracetamol.
Keywords: Dicyclomine Hydrochloride (DCL), Mefenamic Acid (MEFE), Paracetamol (PARA), Simultaneous estimation, Validation.
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