EVALUATION OF ANTIMICROBIAL AND ANTIOXIDANT ACTIVITY OF UNRIPE AND HALF RIPE AEGLE MARMELOS CORR. FRUITS
Deepika Gupta*, PP John, Pankaj Kumar, Dr. Jainendra Jain, Rahul Kaushik, Prof. Manoj Kumar Gupta
ABSTRACT
This study was undertaken to examine the antioxidant and
antimicrobial activity of methanolic extract of Aegle marmelos unripe
or half ripe fruits. The antioxidant activity was done by using DPPH
free radical scavenging assay. The IC50 (The concentration of sample
required to scavenge 50% of DPPH free radical) was calculated by
plotting graph between % inhibition vs concentration. The ascorbic
acid was used as standard antioxidant in comparison to methanolic
extract of Aegle marmelos. The IC50 value of extract and ascorbic acid
was found to be 62.59 μg/ml and 2.80 μg/ml. The antimicrobial
activity was performed using two strains of bacteria E. coli and S.
aureus. The minimum inhibitory concentration (MIC) of the extract
was taken out against two strains and was compared with the inhibition
of standard drug tetracycline. From the results it may be concluded that the methanolic
extract of Aegle marmelos has significant activity against E. coli and S. aureus, the average
inhibition of the test drug against E. coli is 0.43 which is comparable with the inhibition of
tetracycline, 0.954. Again the average inhibition of test drug was found to be 0.49 which was
again comparable with the standard, which showed its median inhibition of 0.965 against S.
aureus. The antioxidant and antimicrobial activities found in Aegle marmelos may be associated with
their main phytochemical compounds like flavonoids, phenols and tannins. This gives the
support that the fruit can be used as the medicinal plant to treat free radical damages in cells
and could also be used as medicinal supplement which could we used with other drugs to
give synergistic effect, as it showed sufficient antibacterial activity at high concentration as
compared to standard drug tetracycline.
Keywords: flavonoids, phenols and tannins.
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