SPECTROPHOTOMETRIC DETERMINATION OF FE (II) AND FE (III) IN PRESENCE OF MICELLAR MEDIUM USING ORTHOHYDROXY BENZAMIDE IN PHARMACEUTICAL SAMPLES
Dr.D.Gopala Krishna* and .Ch.Kethani Devi
ABSTRACT
Two simple, sensitive, rapid and selective spectrophotometric methods
have been developed for the determination of Fe (II) and Fe (III) by
using newly synthesized reagent Ortho-Hydroxy Benzamide (SA) in
presence of acidic surfactant C-TAB (5%). Fe (II) and Iron Fe (III)
forms blue coluored water-soluble complex with SA at pH 5.0 and 5.5
and the complexes shows the maximum absorbance at max 400 and
420 nm for Fe (II) and Fe (III) respectively. At these wavelengths
(max), the complex shows maximum absorbance while the reagent
blanks shows negligible absorbance. Beer's law was obeyed in the
range 0.139-1.396 μg/ml and the optimum concentration range from
ringbom plot was 0.279-1.256 g/ml for Fe (II) and Fe (III)
respectively. The molar absorptivity and Sandell's sensitivity for the
coloured solution were found to be 3.88x104 L mol-1cm-1 and 0.0018-μg.cm-2 for Fe (II) and
4.81x104 L mol-1 cm-1 and 0.00116-μg.cm-2 for Fe (III) respectively. The interference effects
of various diverse ions have been studied. Fe (II) and Fe (III) forms 1:1 complex with SA
stoichiometry with stability constant 3.4x105 for Fe (II) and 3.5x105 for Fe (III). The standard
deviation in the determination of 0.558-μg ml-1 of Fe (II) and Fe (III) was found to be 0.0013
and 0.01 respectively. First and second order derivative Spectrophotometric methods was
developed at max 420, 440 nm for Fe (II) and at 440, 470 nm for Fe (III) which is more
sensitive than the zero order method. The developed method has been employed for the determination of Fe (II) and Fe (III) in Synthetic Alloy samples. The results are in good
agreement with the certified values.
Keywords: Fe (II) & (III), Spectrophotometry, Synthetic Alloy samples, Micellar medium, CTAB (5%), SA.
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