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Abstract

DEVELOPMENT AND VALIDATION OF RP-HPLC METHOD FOR SIMULTANEOUS ESTIMATION OF METHYLDOPA AND HYDROCHLOROTHIAZIDE IN BULK FORM

Khan Hajera*, Mangulkar Ankita, Khan Sana, Mohammad Zameeruddin, Bharkad Vishvanath

ABSTRACT

Present work concerns with development of high performance liquid chromatographic (HPLC) method development for simultaneous determination of Methyldopa and Hydrochlorothiazide in combined dosage form. Chromatographic separation was achieved isocratically on C18 Column Zorbax Eclipse XDB(4.6×250mm×5μ) utilizing Mobile phases consists of Methanol: Water (30:70 v/v) with flow rate of 1ml/min, with detection of 267nm. The Retention Time for Methyldoa & Hydrochlorothiazide was found to be 2.420min and 3.220 min respectively. The developed method was validated in terms of recovery, precision, Ruggedness, Robustness, linearity. This study aimed at developing and validating an HPLC method.

Keywords: Methyldopa, Hydrochlorothiazide, RP- HPLC, Validation.


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