Photo Gallery

Login

Search

News & Updation

  • WJPPS OCTOBER ISSUE PUBLISHED
  • OCTOBER 2017 Issue has been successfully launched on 1 October 2017

  • ICV
  • WJPPS Rank with Index Copernicus Value 52.51 due to high reputation at International Level

  • WJPPS Impact Factor
  • Its our Pleasure to Inform you that WJPPS Impact Factor has been increased from 6.041 to 6.647 due to high quality Publication at International Level

  • Call for Paper
    • WJPPS  Invited to submit your valuable manuscripts for Coming Issue.
  • Updated Version
  • WJPPS introducing updated version of OSTS (online submission and tracking system), which have dedicated control panel for both author and reviewer. Using this control panel author can submit manuscript
  • Journal web site support Internet Explorer, Google Chrome, Mozilla Firefox, Opera, Saffari for easy download of article without any trouble.
  •  

Abstract

STABILITY INDICATING RP-HPLC METHOD DEVELOPMENT AND VALIDATION FOR ESTIMATIION OF DEXTROMETHORPHAN SUSPENSION

Richa Patel*, Komal Patel and Ankit Chaudhary

ABSTRACT

One simple, specific, accurate, precise and reproducible method have been developed and validated for the Simultaneous Estimation of Dextromethorphan in suspension. In Reverse Phase High Performance Liquid Chromatography Method the chromatographic system was equipped with Eclipse- XDB C18 (150 X 4.6 mm, 5μm) Agilent as stationary phase and UV detector set at 225 nm, in conjunction with a mobile phase of 0.05M Potassium Dihydrogen Phosphate buffer (pH- 3.0) and Acetonitrile in the ratio of 80:20% v/v (pH 3.0, adjusted with 1% Orthophosphoric acid) at a flow rate of 1.0 ml/min. The described method was linear over a concentration range of 50-150 μg/ml for Dextromethorphan. The retention time of Dextromethorphan was 3.080 min. The % recoveries of the both the drugs were found to be 100.94-101.91% for Dextromethorphan. Method were statistically validated for accuracy, precision, specificity, LOD, LOQ and robustness according to ICH guidelines and can be used for analysis of combined Tablet. In UV spectrophotometric method was a determination using the First Order Derivative Method at 225 nm over the concentration range 0.5-15 μg/ml for Dextromethorphan. Method were statistically validated for accuracy, precision, LOD and LOQ according to ICH guidelines and can be used for analysis of suspension. The propose method enables rapid quantification and simultaneous analysis of drug from syrup without any interference of excipients. So, the method can be used for routine analysis.

Keywords: Dextromthorphan, RP-HPLC Method, Force Degradation, Method Validation.


[Full Text Article]

Call for Paper

World Journal of Pharmacy and Pharmaceutical Sciences (WJPPS)
Read More

Online Submission

World Journal of Pharmacy and Pharmaceutical Sciences (WJPPS)
Read More

Email & SMS Alert

World Journal of Pharmacy and Pharmaceutical Sciences (WJPPS)
Read More