ANTIBACTERIAL ACTIVITIES OF IRAQI PROPOLIS AND ITS ACTIVE COMPONENTS EXTRACTS ON SOME BACTERIAL ISOLATES (IN VITRO STUDY)
*Shaimaa Obead Hasson
ABSTRACT
Background: Propolis (bee glue) is a resinous product that honeybees
collect from living plants and use in construction and adaptation of
their hives. It is extensively used in folk medicine, and a number of
investigations have shown that propolis posses antibacterial, antiviral
and antifungal properties. Aims: In this paper evaluated the
antibacterial activity of propolis and its active component against ten
bacterial pathogens. Methods :The antibacterial effects of propolis
and active component of propolis against 5 Gram-positive and 5 Gramnegative
bacterial isolates, including Staphylococcus aureus marsa,
Staphylococcus epidermidis, Streptococcus mutanus, Enterococcus
feacalis, Streptococcus pneumoniae, , Pseudomonas fluresence,
Escherchia coli, Proteus mirabilis, Proteus vulgaris, and
Acinetobacter, all of them were studied. Results: Antimicrobial activities of crude ethanol
extract of Kerbalaa propolis (EEKP) and active components of propolis at 25% concentration
against bacterial isolates were studied. The results of agar diffusion at 25% concentration
showed that most bacterial isolates were sensitive to EEKP. S. pneumoniae was highly
sensitive to EEKP than other Gram positive and Gram negative bacteria with inhibition zones
was 30mm. all bacterial isolates were highly sensitive to active component of propolis.
Showing the maximum zone of inhibition of 30 mm at the 25% concentration
Conclusions: In summary, the propolis and its active component extract showed a wide
spectrum activity and appears to satisfy all of the criteria for antibacterial agents. These results suggest that propolis can be used to protect food and reduce the risk of contamination
from pathogenic microorganisms.
Keywords: propolis, active component of propolis, and antibacterial activity.
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